Figure 4.

The Fd2‐knockout mutant (Fd2‐KO) displays enhanced expression of jasmonic acid (JA)‐responsive genes and compromised induction of salicylic acid (SA)‐responsive genes on pathogen infection. After inoculation with Pseudomonas syringae pv. tomato (Pst) DC3000 [optical density at 600 nm (OD₆₀₀) = 0.0002] by infiltration, total RNA was extracted from the inoculated leaves of Nossen and Fd2‐KO plants at the indicated times. The relative transcriptional levels of PDF1.2 (a), MYC2 (b), SID2 (c), PR1 (d), PR2 (e) and PR5 (f) were determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) with Actin as the endogenous control. Error bars represent the standard error (SE) (n = 3), and significance was determined at *P < 0.05 and **P < 0.01 with Student's t‐test. Experiments were conducted in triplicate with similar results. dpi, days post‐infection.