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. 2017 Jul 31;19(4):827–840. doi: 10.1111/mpp.12566

Figure 2.

Figure 2

Lon‐dependent degradation of HrpS/HrpA regulates type III secretion system (T3SS) gene expression in Erwinia amylovora. Abundance of HrpS‐His6 (A) and HrpA‐His6 (B) proteins in the wild‐type (WT) and lon mutant strains grown in hrp‐inducing medium (HMM) for 6 h at 18 °C; the abundance of the RpoB protein was used as a loading control. Half‐life of HrpS‐His6 (C) and HrpA‐His6 (D) proteins in the WT and lon mutant strains. For protein stability test, cells were grown in HMM for 6 h at 18 °C. Translation was stopped by the addition of tetracycline, and samples were taken at the indicated time points (min) at the top. The relative protein abundance at the bottom of each lane was calculated using ImageJ software. Cropped gel blots and percentage expression values are representatives of three independent experiments. ND, not detected.