Figure 3.

Lon‐dependent degradation of RcsA regulates amylovoran production in Erwinia amylovora. Abundance of RcsA‐His6 (A) and RcsB‐His6 (B) proteins in the wild‐type (WT) and lon mutant strains grown in MBMA medium for 24 h at 28 °C; the abundance of the RpoB protein was used as a loading control. Half‐life of RcsA‐His6 (C) and RcsB‐His6 (D) proteins in the WT and lon mutant strains. For protein stability test, cells were grown in MBMA medium for 24 h at 28 °C. Translation was stopped by the addition of tetracycline, and samples were taken at the indicated time points (min) at the top. Relative protein abundance at the bottom of each lane was calculated using ImageJ software. Cropped gel blots and percentage expression values are representatives of three independent experiments.