Table 1.
Bacterial strains and plasmids used in this study.
| Strains, plasmids | Description | Reference, source |
|---|---|---|
| Erwinia amylovora | ||
| Ea1189 | Wild‐type, isolated from apple | Wang et al. (2009) |
| Δlon | lon::Cm; CmR‐insertional mutant of lon of Ea1189 | This study |
| ΔrcsA | rcsA::Cm; CmR‐insertional mutant of rcsA of Ea1189 | Ancona et al. (2014) |
| ΔrcsB | rcsB::Km; KmR‐insertional mutant of rcsB of Ea1189 | Wang et al. (2009) |
| Δlon/rcsA | rcsA::Cm, lon::Km; KmR‐insertional mutant of lon of ΔrcsA | This study |
| Δlon/rcsB | rcsB::Km, lon::Cm; CmR‐insertional mutant of lon of ΔrcsB | This study |
| ΔcsrA | csrA::Cm; CmR‐insertional mutant of csrA of Ea1189 | Ancona et al. (2016) |
| ΔcsrB | csrB::Cm; CmR‐insertional mutant of csrB of Ea1189 | Ancona et al. (2016) |
| Δlon/csrA | csrA::Km, lon::Cm; KmR‐insertional mutant of csrA of Δlon | This study |
| Escherichia coli | ||
| DH10B | F– mcrA Δ(mrr‐hsdRMS‐mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ– | Invitrogen (Carlsbad, CA, USA) |
| Plasmids | ||
| pKD46 | ApR, PBAD gam bet exo pSC101 oriTS | Datsenko and Wanner (2000) |
| pKD32 | CmR, FRT cat FRT tL3 oriR6Kγ bla rgnB | Datsenko and Wanner (2000) |
| pKD13 | KmR, FRT kan FRT tL3 oriR6Kγ bla rgnB | Datsenko and Wanner (2000) |
| pWSK29 | ApR, cloning vector, low copy number | Wang and Kushner (1991) |
| pLon | 3077‐bp DNA fragment containing promoter sequence of lon gene in pWSK29 | This study |
| pHrpS‐His6 | 1537‐bp DNA fragment containing promoter sequence of hrpS gene and C‐terminal His‐tag in pWSK29 | This study |
| pHrpA‐His6 | 803‐bp DNA fragment containing promoter sequence of hrpA gene and C‐terminal His‐tag in pWSK29 | Ancona et al. (2015b) |
| pRcsA‐His6 | 1058‐bp DNA fragment containing promoter sequence of rcsA gene and C‐terminal His‐tag in pWSK29 | This study |
| pRcsB‐His6 | 1142‐bp DNA fragment containing promoter sequence of rcsB gene and C‐terminal His‐tag in pWSK29 | This study |
| pLon‐His6 | 2877‐bp DNA fragment containing promoter sequence of lon gene and C‐terminal His‐tag in pWSK29 | This study |