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. 2016 Dec 27;19(1):129–142. doi: 10.1111/mpp.12504

Figure 1.

Figure 1

RipAY‐GFP suppresses immune responses in Nicotiana benthamiana leaves. Agrobacterium tumefaciens was used to induce the transient expression of RipAY‐GFP in one half of the leaf and green fluorescent protein (GFP) in the other half. (a, b) Oxidative burst triggered by 50 nm flg22 in N. benthamiana tissues at 2.5 days post‐inoculation (dpi) with A. tumefaciens, and measured in a luminol‐based assay as relative luminescence units (RLU). Values are the average ± standard error (SE) (n = 24). (c) Quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of NbPR1 transcripts in N. benthamiana leaves, either untreated or treated with 1 mm salicylic acid (SA) for 12 h, at 2.5 dpi with A. tumefaciens. Gene expression values are relative to the NbEF1α housekeeping gene and are normalized to untreated N. benthamiana tissues. Values are the average ± SE (n = 3). Asterisks indicate significant differences compared with the corresponding GFP control at P < 0.001. The experiments were repeated at least three times with similar results.