Skip to main content
. 2017 Feb 16;19(2):381–392. doi: 10.1111/mpp.12528

Figure 1.

Figure 1

(A) Schematic structure of PthG. (B) Translocation cassette for the translocation assay of type III effectors (T3Es) into beet cubes. The translocation cassette consists of multiple cloning sites (MCSs) and the C‐terminus of PthG containing the hypersensitive response (HR) domain fused to a His‐Tag. The translocation cassette was cloned into pVSP61 under the lac promoter to yield kanamycin (Km)‐resistant pVSP‐PtHis. aa, amino acid. Details of the assay procedure are described in the Results section.