Table 4.
Virulence of Pab4188* | Virulence of Pag824‐1* | ||||
---|---|---|---|---|---|
ORF | Presence in Pab | Presence in Pag | Gypsophila | Beet | Gypsophila |
T3Es | |||||
HsvG † | + | + | H | None | H |
HsvB † | + | + | None | H | None |
PthG | + (truncated) | + | None | None ‡ | H |
PseB | + | ‐ | H | M | None ‡ |
DspA/E | + | + | H | M | H |
HopAY1 | + | + | M | M | L |
HopD1 | + (truncated) | + | None ‡ | None ‡ | L |
HopX2 | + | + | M | M | L |
HopAF1 | + | + | M | M | L |
HrpK | + | + | M | M | L |
Harpins | |||||
HopAK1 | + | + | None | None | None |
HrpN | + | + | None | None | None |
*Virulence of the corresponding type III effector mutant expressed as the percentage reduction in gall fresh weight relative to the wild‐type. Degrees of contribution to virulence are as follows: high (H), above 70% reduction in gall size; medium (M), between 21% and 69% reduction; low (L), below 20% reduction; None, no effect on virulence. Results are the average of three independent experiments. Average gall size for Pab4188 or Pag824‐1 in gypsophila is 202 ± 12 mg, and for Pab4188 in beet is 171 ± 10 mg.
†Gall reduction in mutants was 90% or above.
‡Partial sequence (PthG and HopD1 in Pab) or completely deleted (PseB in Pag).