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. 2017 Dec 20;19(6):1319–1331. doi: 10.1111/mpp.12615

Figure 5.

Figure 5

Cell‐to‐cell movement of tomato apex necrosis virus (ToANV) wild‐type (WT) RNA2‐GFP vector and RNA2‐MP‐ and RNA2‐ORF1‐vector mutants in Nicotiana benthamiana epidermal tissue. Agrobacterium tumefaciens suspensions were agroinfiltrated in serial dilutions (1 : 50 to 1 : 5000) into leaves of N. benthamiana plants starting from a suspension adjusted to 0.25 absorbance at 600 nm for each treatment. Groups of cells expressing green fluorescent protein (GFP) were evident with the constructs encoding GFP either from the WT RNA2 (R1+R2‐GFP, E to H) or from the RNA2‐ORF1 deletion mutant (R1+R2‐Δp21‐GFP, M to P), suggesting that cell‐to‐cell movement occurred even in the absence of the RNA2‐ORF1 protein. Single‐cell transfection was obtained with the negative controls pBIN‐GFP (A to D) and the RNA2‐MP‐vector mutant (R1+R2‐ΔMP‐GFP, I to L) at 1 : 5000 dilution confirming that ToANV movement protein (MP) is required for cell‐to‐cell movement in N. benthamiana plants. (D), (H), (L) and (P) are enlargements of the areas marked by white square boxes in (C), (G), (K) and (O), respectively. Bars, 100 µm.