Figure 6.

Western blot analyses of Nicotiana benthamiana plants agroinfiltrated with constructs initiating infection of tomato apex necrosis virus (ToANV) wild‐type (WT), R2‐GFP, R2‐ΔMP‐GFP and R2‐Δp21‐GFP using anti‐ToANV‐capsid protein (CP) antibodies. Western blots from leaves of N. benthamiana plants agroinfiltrated with ToANV binary constructs R1+R2‐GFP (A and B), R1+R2‐Δp21‐GFP (A) and R1+R2‐ΔMP‐GFP (B). Agrobacterium tumefaciens suspensions were infiltrated in serial dilutions from 1 : 40 to 1 : 4000 into leaves of N. benthamiana. Coomassie blue staining of the loading controls is shown in the bottom panel. Each lane represents one biological replicate. L1 and L2 refer to two different leaves from the same treatment. Each half leaf was inoculated with the treatment indicated above each lane.