Figure 8.

Immunolocalization of tomato apex necrosis virus (ToANV) capsid protein (CP) in cross‐sections of petioles (A to D) and leaf veins (E to P) of Nicotiana benthamiana plants agroinfiltrated with constructs expressing ToANV wild‐type (WT) (R1+R2) and RNA2‐ORF1 protein mutant (R1+R2‐Δp21). We show images representative of two distinct experiments performed in four distinct agroinfiltrated areas for each construct. Ten sections were observed for at least one nervature in each agroinfiltrated area. Images are the overlays of bright field and fluorescent signals of fluorescein isothiocyanate conjugate, specific for ToANV CP detection. (F), (H), (J), (L), (N) and (P) are enlargements of the areas marked by white square boxes in (E), (G), (I), (K), (M) and (O), respectively. abe, abaxial epidermis; ade, adaxial epidermis; pa, parenchyma; ph, phloem; x, xylem. Scale bars: white, 100 μm; black, 20 μm.