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. 2018 Sep 28;19(11):2384–2396. doi: 10.1111/mpp.12717

Figure 3.

Figure 3

The leaf yellowing symptom was also attenuated when nbe‐miR166h‐p5 was suppressed by Potato virus X (PVX)‐mediated virus‐based microRNA (miRNA) suppression (VbMS). (A) PVX itself was used as a tool to suppress nbe‐miR166h‐p5 through a VbMS system. At 8 days post‐inoculation (dpi), nbe‐miR166h‐p5‐suppressed plants (PVX:TM) showed attenuated symptoms compared with the control (PVX:00). (B) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) showed that suppressed plants (PVX:TM) had 5% of the level of nbe‐miR166h‐p5 present in control plants (PVX:00). (C) The chlorophyll content in nbe‐miR166h‐p5‐suppressed leaves (PVX:TM) was significantly higher than that in control leaves (PVX:00). (D) Western and northern blotting showed that PVX accumulated at a lower level in both inoculated and systemic leaves in nbe‐miR166h‐p5‐suppressed plants. CP, coat protein. Bars represent the standard errors of the means. A two‐sample unequal variance directional t‐test was used to test the significance of the difference (**P < 0.01). [Colour figure can be viewed at wileyonlinelibrary.com]