Figure 7.
Regulation of the REPBaMV activity by NbMKP1. Cell extracts of N. benthamiana leaves, harvested on day 2 after agroinfiltration with binary plasmids pERep‐HA and pKSF4, in combination without or with plasmid pEpNbMKP1 or pEpNbMKP1(C201S), were subjected to centrifugation at 30000×g. The pellet (P30) after centrifugation were analyzed by (A) western blotting for the detection of REPBaMV‐HA and (B) in vitro RNA‐dependent RNA polymerase activity assay using the endogenous satellite RNA SF4 as the template. The statistical data are the means of three independent experiments.