Table 1.
Phenotype assay of the strains used in this study.
| Strain | Sorbitol (2 m) | NaCl (1 m) | SDS (0.02%) | SDS (0.01%) | K2S2O8 (30 mm) | K2S2O8 (20 mm) | Tebuconazole (0.5 µg/mL) | Germinated conidia (%) |
|---|---|---|---|---|---|---|---|---|
| FcM7 | 2.30 ± 0.04 | 5.26 ± 0.02 | 2.14 ± 0.02 | N.D. | 2.56 ± 0.14 | N.D. | 5.26 ± 0.02 | 0.74 ± 0.01 |
| R38 | 0.84 ± 0.02** | 0.82 ± 0.02** | 1.14 ± 0.02** | N.D. | 0.70 ± 0.0** | N.D. | 1.58 ± 0.02** | 0.77 ± 0.01 |
| Fusarium culmorum UK99 | 2.98 ± 0.02 | 4.88 ± 0.05 | 2.78 ± 0.02 | N.D. | No growth | 4.87 ± 0.07 | 4.13 ± 0.14 | 0.30 ± 0.02 |
| FcB6 (ectopic) | 2.68 ± 0.10 | 5.02 ± 0.05 | 2.90 ± 0.03 | N.D. | No growth | 5.10 ± 0.06 | 4.33 ± 0.05 | 0.28 ± 0.01 |
| ΔFcRav2 B24 | 1.50 ± 0.07** | 1.40 ± 0.03 | 1.80 ± 0.03** | N.D. | No growth | 2.50 ± 0.0** | 2.77 ± 0.03** | 0.05 ± 0.01** |
| ΔFcRav2 B51 | 1.56 ± 0.05** | 1.80 ± 0.09 | 1.86 ± 0.02** | N.D. | No growth | 2.77 ± 0.03** | 3.13 ± 0.05** | 0.02 ± 0.01** |
| Fusarium graminearum PH1 | 4.02 ± 0.04 | 4.86 ±0.04 | No growth | 4.83 ± 0.03 | No growth | 5.00 ± 0.0 | 5.10 ± 0.10 | 0.34 ± 0.02 |
| ΔFgRav2 G8 | 1.08 ± 0.06** | 2.84 ± 0.05** | No growth | 3.26 ± 0.03** | No growth | 3.97 ± 0.07** | 2.08 ± 0.08** | 0.14 ± 0.01** |
| ΔFgRav2 G10 | 1.6 ± 0.09** | 3.78 ± 0.05** | No growth | 3.13 ± 0.08** | No growth | 3.70 ± 0.15** | 2.36 ± 0.05** | 0.15 ± 0.01** |
Colony growth was measured after 3 days of incubation at 25 °C on Czapek dox agar supplemented with 2 m sorbitol, 1 m NaCl, 0.01%–0.02% (w/v) sodium dodecylsulfate (SDS) (osmotic stress), 20–30 mm K2S2O8 (oxidative stress) or 0.5 µg/mL tebuconazole (fungicide sensitivity). Percentage spore germination was evaluated after 4 h of incubation in Czapek dox broth. Three independent tests were performed with three replicates for each test.
Results are expressed as colony diameter growth (cm) ± standard error. Significantly different from the control (**P < 0.01) based on Dunnett's test.
N.D., not determined.