Figure 5.

Prolines are preferentially excluded from the first 20% of proline‐rich pioneers. On average, the proline content of the proline‐rich effectors is non‐evenly distributed across the open reading frame, and preferentially excluded from the first 1%–20% (black). This is in stark contrast with all Pratylenchus penetrans predicted proteins (light blue), transcripts that encode putatively secreted proteins (light grey) and all transcripts expressed in the gland cells (dark grey). Five proteins were selected at random in each of 250 iterations. In each iteration, the average distribution of prolines in those five proteins was calculated. The means of all 250 iterations are shown, with error bars indicating the standard deviation.