Table 2.
Summary of Pratylenchus penetrans gene transcripts without functional known annotation selected for in situ hybridization assays.
| Tylenchida | Aph. | Rhab. | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P. penetrans | Pratylenchidae | RKN | Cyst | ||||||||||||
| Transcript code | Gland dataset (n = 24) | In planta dataset (n = 67) | Pc (n = 18) | Pz (n = 11) | Pt (n = 4) | Pv (n = 5) | Rs (n = 3) | Mi (n = 9) | Mh (n = 10) | Gp (n = 5) | Gr (n = 4) | Dd (n = 5) | Bx (n = 1) | Ce (n = 0) | ANNOTATION – NR database |
| Ppen12587_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11402_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12898_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen3243_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13578_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13114_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen9482_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen14240_c2_seq1 | – | Yes | + | + | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13485_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen16416_c0_seq1 | Yes | Yes | + | + | + | + | – | + | + | – | – | – | – | – | – |
| Ppen8004_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12088_c0_seq1 | – | Yes | – | – | – | – | + | – | + | – | – | – | – | – | – |
| Ppen7984_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11964_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen17512_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11603_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11206_c0_seq1 | – | Yes | + | + | – | – | – | – | – | – | – | – | – | – | – |
| Ppen16605_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12016_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13388_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen14681_c0_seq1 | – | Yes | + | + | + | – | + | – | + | + | + | + | + | – | – |
| Ppen11135_c0_seq1 | – | Yes | + | – | – | + | – | + | – | – | – | – | – | – | – |
| Ppen3331_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen15637_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13090_c0_seq1 | – | Yes | + | + | – | – | – | + | + | + | + | – | – | – | – |
| Ppen14446_c0_seq1 | – | Yes | + | + | + | – | – | + | + | + | – | + | – | – | – |
| Ppen12616_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen14188_c0_seq1 | – | Yes | + | + | – | + | + | + | + | + | + | + | – | – | – |
| Ppen8861_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13037_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen18978_c0_seq1 | – | Yes | – | – | – | – | – | – | + | – | – | – | – | – | – |
| Ppen10370_c0_seq1 | – | Yes | + | + | + | – | – | – | – | – | – | – | – | – | – |
| Ppen10414_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen8129_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen9159_c0_seq1 | – | Yes | – | – | – | – | – | + | + | – | – | – | – | – | – |
| Ppen5003_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11094_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen15969_c0_seq1 | – | Yes | + | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen14399_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen10237_c0_seq1 | – | Yes | + | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen16124_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen9671_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11230_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen15066_c0_seq1 | Yes | Yes | + | + | + | – | – | + | + | + | + | + | – | – | – |
| Ppen14417_c0_seq1 | Yes | Yes | + | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen16557_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12211_c0_seq1 | – | Yes | – | – | – | + | – | – | – | – | – | – | – | – | – |
| Ppen12633_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12501_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11421_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen5669_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen18231_c0_seq1 | Yes | Yes | + | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13380_c0_seq1 | Yes | Yes | + | – | – | – | – | + | – | – | – | – | – | – | – |
| Ppen11641_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12399_c0_seq1 | – | Yes | + | + | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12805_c0_seq1 | – | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen18503_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen11677_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12366_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen8205_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen19523_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen13553_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12216_c0_seq1 | – | Yes | + | + | – | + | – | + | + | – | – | + | – | – | – |
| Ppen12120_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen16911_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen10194_c0_seq1 | Yes | Yes | + | – | – | – | – | – | – | – | – | – | – | – | – |
| Ppen12271_c0_seq1 | Yes | Yes | – | – | – | – | – | – | – | – | – | – | – | – | – |
blast searches were performed against sequences in the non‐redundant (NR) database at the National Center for Biotechnology Information (NCBI) for a putative annotation, and against specific nematode proteins or transcriptome datasets for the presence/absence of positive blast hits (e‐value cutoff of 1e‐5 and bitscore > 50) in the corresponding species.
n represents the total number of genes with a positive blast hit against the 67 genes of P. penetrans.
Aph., Aphelenchida; Bx, Bursaphelenchus xylophilus; Ce, Caenorhabditis elegans; Cyst, cyst nematodes (Heteroderidae); Dd, Ditylenchus destructor; Gp, Globodera pallida; Gr, G. rostochiensis; Mh, Meloidogyne hapla; Mi, M. incognita; Pc, Pratylenchus coffeae; Pt, P. thornei; Pv, P. vulnus; Pz, P. zeae; Rhab., Rhabditida; RKN, root‐knot nematode (Meloidogynidae); Rs, Radopholus similis.