Table 3.
Characterization of corresponding predicted protein sequences whose gene transcripts were specifically localized in the oesophageal glands of Pratylenchus penetrans
| PSORTII prediction | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Transcript code | Interpro accession | InterPro name | InterPro signatures | Protein (amino acid) | Domain position | Domain bit score | e‐value | Proline content (%) | Subcellular localization | Probability |
| Homologues of known effector genes or genes with relevant annotation | ||||||||||
| Ppen15842_c0_seq1 | IPR001547 | Glycoside hydrolase family 5 | PF00150 (PFAM) | 457 | 44–290 | 180.8 | 3.00E‐53 | 3.9 | Cytoplasmic | 60.9 |
| Ppen16218_c0_seq1 | IPR001547 | Glycoside hydrolase family 5 | PF00150 (PFAM) | 446 | 36–289 | 145 | 2.70E‐42 | 3.8 | Cytoplasmic | 47.8 |
| Ppen13447_c0_seq1 | IPR004898 | Pectate lyase catalytic | PF03211 (PFAM) | 260 | 19–236 | 214.1 | 1.50E‐63 | 1.2 | Nuclear | 56.5 |
| Ppen14256_c0_seq1 | IPR004898 | Pectate lyase catalytic | PF03211 (PFAM) | 264 | 20–257 | 86.4 | 1.70E‐24 | 3.8 | Nuclear | 73.9 |
| Ppen12533_c0_seq1 | IPR009009 | RlpA‐like protein double‐psi beta‐barrel domain | PF03330 (PFAM) | 180 | 56–73 | 28.8 | 8.70E‐07 | 5.6 | Nuclear | 34.8 |
| Ppen15554_c1_seq1 | IPR001919,IPR009009 | Carbohydrate‐binding type‐2 domain, RlpA‐like protein double‐psi beta‐barrel domain | PF00553 (PFAM), PF03330 (PFAM) | 323 | 25–118, 202–316 | 32 | 31.2 | 9.0E‐7, 2.1E‐8 | 6.8 | Nuclear | 39.1 |
| Ppen18759_c0_seq1 | IPR011683 | Glycosyl hydrolase family 53 | PF07745 (PFAM) | 336 | 27–283 | 296.7 | 1.80E‐88 | 3.6 | Cytoplasmic | 60.9 |
| Ppen12597_c1_seq1 | IPR033452,IPR033453 | Glycosyl hydrolase family 30 beta sandwich domain, glycosyl hydrolase family 30 TIM‐barrel domain | PF17189 (PFAM), PF02055 (PFAM) | 400 | 49–187 | 36.9 | 2.20E‐09 | 3.5 | Cytoplasmic | 47.8 |
| Ppen11632_c0_seq1 | IPR014044 | CAP domain | PF00188 (PFAM) | 212 | 35–174 | 59.2 | 1.30E‐20 | 2.4 | Nuclear | 39.1 |
| Ppen15229_c0_seq1 | IPR001580 | Calreticulin/calnexin | PF00262 (PFAM) | 412 | 23–333 | 206.9 | 9.80E‐123 | 5.6 | Endoplasmic reticulum | 55.6 |
| Ppen16493_c0_seq1 | IPR011614,IPR010582 | Catalase core domain, catalase immune‐responsive domain | PF00199 (PFAM), PF06628 (PFAM) | 512 | 44–425, 445–511 | 616.7 | 49.5 | 9.6E‐176, 9.5E‐14 | 7 | Cytoplasmic | 52.2 |
| Ppen13849_c0_seq1 | IPR002919 | Trypsin inhibitor‐like cysteine‐rich domain | PF01826 (PFAM) | 151 | 37–91 | 40.8 | 1.80E‐10 | 11.9 | Nuclear | 78.3 |
| Ppen12895_c0_seq1 | IPR008632 | Nematode fatty acid retinoid binding | PF05823 (PFAM) | 188 | 31–180 | 84.5 | 4.80E‐24 | 4.3 | Nuclear | 47.8 |
| Ppen12103_c0_seq1 | IPR003677 | Domain of unknown function DUF148 | PF02520 (PFAM) | 209 | 54 | 149 | 7.00E‐15 | 14.4 | Nuclear | 69.6 |
| Pioneer candidate effectors | ||||||||||
| Ppen11402_c0_seq1 | – | – | – | 79 | – | – | – | 6.3 | Cytoplasmic | 69.6 |
| Ppen8004_c0_seq1 | – | – | – | 92 | – | – | – | 23.9 | Nuclear | 65.2 |
| Ppen7984_c0_seq1 | – | – | – | 73 | – | – | – | 25.7 | Nuclear | 56.5 |
| Ppen16605_c0_seq1 | – | – | – | 102 | – | – | – | 22.5 | Nuclear | 43.5 |
| Ppen12016_c0_seq1 | – | – | – | 129 | – | – | – | 20.9 | Nuclear | 60.9 |
| Ppen10370_c0_seq1 | – | – | – | 101 | – | – | – | 13.9 | Nuclear | 39.1 |
| Ppen11230_c0_seq1 | – | – | – | 176 | – | – | – | 7.4 | Nuclear | 60.9 |
| Ppen15066_c0_seq1 | IPR003677 | Domain of unknown function DUF148 | PF02520 (PFAM) | 590 | 266–372 | 29.7 | 4.90E‐07 | 7.3 | Cytoplasmic | 69.6 |