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. 2018 Apr 16;19(8):1917–1928. doi: 10.1111/mpp.12668

Figure 3.

Figure 3

Overexpression of HIPP27 leads to increased susceptibility to Heterodera schachtii. (A) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) confirmation of the increase in HIPP27 transcript in overexpression lines of the Col‐0 (OXHIPP27) or hipp27a (OXHIPP27/hipp27a) background (n = 3). (B, C) Average sizes of syncytia (B) and female nematodes (C) in Col‐0 (n = 77) and HIPP27 (OXHIPP27/L1, n = 75; OXHIPP27/L2, n = 68; OXHIPP27hipp27a/L1, n = 90; OXHIPP27hipp27a/L2, n = 75) overexpression lines at 14 days post‐infection (dpi). (D) Average number of nematodes per plant present in Col‐0 (n = 40) and HIPP27 overexpression lines (OXHIPP27/L1, n = 24; OXHIPP27/L2, n = 28; OXHIPP27hipp27a/L1, n = 26; OXHIPP27hipp27a/L2, n = 24) at 14 dpi. (A–D) Bars represent mean ± standard error (SE). Asterisks denote significant difference from corresponding Col‐0 value (t‐test, *P < 0.05).