Table 1.
Validation of the expression of 10 selected candidate genes in syncytia induced in Arabidopsis roots by beet cyst nematode.
| Fold change compared with uninfected control | |||
|---|---|---|---|
| Gene | Locus | Microarrays (5 + 15 dpi) | qRT‐PCR (15 dpi) |
| ENH1 | AT5G17170 | 27.9 | 3.7 ± 0.26 |
| UGP2 | AT5G17310 | 29.9 | 2.6 ± 0.56 |
| – | AT4G24830 | 29.9 | 3.3 ± 0.59 |
| IMD2 | AT1G80560 | 32.0 | 7.3 ± 4.7 |
| NPC6 | AT3G48610 | 39.3 | 30.0 ± 11.6 |
| AD11A3 | AT2G24270 | 48.5 | 44.5 ± 16.9 |
| TRX1 | AT3G51030 | 48.5 | 11.2 ± 3.28 |
| CCR2 | AT1G80820 | 52.0 | 15.47 ± 3.48 |
| FAD6 | AT4G30950 | 52.0 | 5.04 ± 1.71 |
| HIPP27 | AT5G66110 | 68.6 | 24.81 ± 2.88 |
For microarrays, data from microaspirated syncytia at 5 and 15 days post‐infection (dpi) were pooled and compared with control roots (Szakasits et al., 2009). For quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR), values represent the relative fold change in infected root segment containing syncytia relative to control uninfected root. 18S and UBP22 were used as housekeeping genes to normalize the data. All values are the means of three biological replicates ± standard error (SE)