Figure 2.
Verification of interaction between Fsr1 and FvStp1 interaction in vivo. (A) The pair of plasmids pGADT7‐FSR1 and pGBKT7‐FvSTP1 was used to test interaction by monitoring blue colonies that developed on Synthetic defined medium without Ade‐His‐Leu‐Trp (Clontech) amended with X‐alpha‐Gal. The pair of plasmids pGBKT7–53 and pGADT7 was used as a positive control. The plasmid pairs pGBKT7‐Lam/pGADT7, pGBKT7‐FSR1/pGADT7, and pGBKT7‐STP1/pGADT7 were used as negative controls. (B) Further verification of in vivo interactions between Fsr1 and FvStp1 was tested by split luciferase complementation assay. Fcc1/Fck1 was used as positive control, and six negative controls were tested (including no vector [NA]). Luminescence activity was obtained from three replicates of fungal inoculations and is presented as the number of RLUs (relative light units) per 106 fungal conidia. Asterisk above the column means statistically significant (P<0.05) analyzed by t‐Test.