Figure 7.

Mutants of the Xanthomonas oryzae pv. oryzae (Xoo) chemotaxis component exhibit altered biofilm formation. (A) Biofilm formation by different Xoo strains in the static biofilm after 48 h of growth and staining with 0.1% crystal violet. (B) Quantification of attached cells of different Xoo strains in the static biofilm after 48 h of growth. Attached cells were stained with crystal violet, dissolved in ethanol and quantified by measurement of the absorbance at 570 nm. Data are shown as the mean ± standard deviation (SD) (n = 3). *^,**Statistically significantly different values compared with the wild‐type strain (Student’s t‐test; *P < 0.001, **P < 0.01). The experiment was repeated three times. [Colour figure can be viewed at wileyonlinelibrary.com]