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. 2017 May 18;19(3):700–714. doi: 10.1111/mpp.12555

Figure 9.

Figure 9

Transient overexpression of GmRBOH‐VI in Nicotiana benthamiana leads to enhanced susceptibility to Sclerotinia sclerotiorum. (A) Detection of GmRBOHB‐HA, GmRBOHL‐HA, GmRBOHP‐HA and GmRBOHQ‐HA from infiltrated N. benthamiana leaves. The pGWB414‐GmRBOHB‐HA, pGWB414‐GmRBOHL‐HA, pGWB414‐GmRBOHP‐HA, pGWB414‐GmRBOHQ‐HA and pGWB414‐eHA (empty vector) constructs were expressed in leaves by Agrobacterium infiltration, and samples were collected at 48 h post‐infiltration. Total soluble protein extracts were prepared and separated using sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and tagged GmRBOH proteins were detected using a haemagglutinin (HA)‐specific antibody. Equal loading of protein samples was confirmed by Ponceau staining. (B) Lesion area. pGWB414‐GmRBOHB‐HA, pGWB414‐GmRBOHL‐HA, pGWB414‐GmRBOHP‐HA, pGWB414‐GmRBOHQ‐HA and pGWB414‐eHA were expressed in N. benthamiana leaves using Agrobacterium. At 24 h post‐infiltration, leaves were detached and challenged with S. sclerotiorum. The lesion diameter was measured at 24 h post‐inoculation (hpi). (C) Lesion development in representative leaves. Mean lesion area ± standard deviation (SD) from three independent experiments was measured; each experiment contained five leaves. ⋆, Significant difference at P < 0.05.