Table 2.
List of bacterial strains and plasmids used in this study.
| Bacterial strain/plasmid | Relevant characteristic(s) | Reference/source |
|---|---|---|
| Escherichia coli strains | ||
| DH5α | λ– f80d lacZDM15 D(lacZYA‐argF) U169 recA1 endA hsdR17 ( ) supE44 thi‐1 gyrA relA1 | Invitrogen, Carlsbad, CA, US |
| S17‐1 | RP4‐2 Tc::Mu‐Km::Tn7 pro hsdR recA Tra+ used as mobilizing strain | Simon et al. (1983) |
| Xanthomonas oryzae pv. oryzae strains | ||
| BXO1 | Wild‐type; Indian isolate | Laboratory collection |
| BXO43 | rif‐2; derivative of BXO1 | Laboratory collection |
| ΔcbsA | rif‐2; derivative of BXO43 | This study |
| ΔcbsA/pHM1 | ΔcbsA/pHM1; rif‐2; Spr derivative of ΔcbsA | This study |
| ΔcbsA/pHM1::cbsA | ΔcbsA/pHM1::cbsA; rif‐2; Spr derivative of ΔcbsA | This study |
| ΔcbsA/pHM1::D131AcbsA | ΔcbsA/pHM1::D131AcbsA; rif‐2; Spr derivative of ΔcbsA | This study |
| T3SS– | hrpB6::bla rif‐2; HR–, Apr derivative of BXO43 | Jha et al. (2007) |
| T3SS– cbsA– | ΔcbsA:: rif‐2; Apr derivative of T3SS– | Tayi et al. (2016a) |
| T3SS– cbsA–/pHM1 | T3SS– cbsA–/pHM1; rif‐2; Apr, Spr; derivative of T3SS– cbsA– | Tayi et al. (2016a, b) |
| T3SS– cbsA–/pHM1::cbsA | T3SS– cbsA–/pHM1::cbsA; rif‐2; Apr, Spr; derivative of T3SS– cbsA– | This study |
| T3SS– cbsA–/pHM1::D131AcbsA | T3SS– cbsA–/pHM1::D131AcbsA; rif‐2; Apr, Spr; derivative of T3SS– cbsA– | This study |
| Plasmids | ||
| pK18mobsacB | Allelic exchange suicide vector SacB derivative of pK18mob; sacB Tra–Mob+, Kmr does not replicate in X. oryzae pv. oryzae | Schäfer, Tauch et al. (1994) |
| pTL1 | pK18mobsacB+1373 bp of A+C fragment of cbsA gene | Tayi et al. (2016a, b) |
| pBSKS | Cloning vector; Apr | Stratagene |
| pBSKS Wt CbsA | pBSKS + 1701 bp cbsA | This study |
| pBSKS D131A CbsA | pBSKS + 1701 bp D131AcbsA | This study |
| pHM1 | Broad‐host‐range cosmid vector (13.3 kb); Spr | Innes, Hirose et al. (1988) |
| pHM1 Wt CbsA | pHM1 + 1701 bp cbsA gene | This study |
| pHM1 D131A CbsA | pHM1 + 1701 bp D131AcbsA gene | This study |