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. 2017 Dec 26;19(6):1343–1352. doi: 10.1111/mpp.12617

Figure 3.

Figure 3

Up‐regulation of defence‐related features in silenced wheat plants without Blumeria graminis f. sp. tritici (Bgt) infection at 22 °C. (A, B) RNA expression of pathogenesis‐related genes TaPR2 (A) and TaPR10 (B) analysed by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in BSMV:00‐, BSMV:TaBON1‐ and BSMV:TaBON3‐inoculated plants at 22 °C. RNA samples were isolated from the fourth leaves of wheat seedlings. Expression is normalized by the expression level in the control (BSMV:00). TaActin was used as the internal control. Similar results were seen in all three biological replicates and one biological replicate with three technical repeats is shown. Asterisks indicate statistically significant differences in comparison with the control (BSMV:00) at P ≤ 0.01 (Student's t‐test). (C, D) The fourth leaves at 14 days before challenge with Bgt were stained with 3,3′‐diaminobenzidine (DAB) (C) and trypan blue (D). Scale bars, 1 cm. Three plants for TaBON1 and TaBON3 silencing were analysed.