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. 2017 Dec 26;19(6):1343–1352. doi: 10.1111/mpp.12617

Figure 5.

Figure 5

Temperature effects on the expression of TaBON1 and TaBON3, as well as defence induced by the silencing of TaBON1 and TaBON3. (A, B) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of TaBON1 (A) and TaBON3 (B) relative expression levels at different temperatures in common wheat leaves. RNA samples were isolated from the leaves of high‐temperature‐treated common wheat Sumai 3 at 22, 25 and 32 °C. Expression was normalized by the expression level at 22 °C. (C) The leaf phenotype of successful virus infection. Photographs were captured at 8 days after virus inoculation. MOCK: wheat leaves treated with 2 × GKP buffer. Scale bars, 1 cm. (D, E) Relative transcript levels of TaBON1 (D) and TaBON3 (E) in plants at 28 °C inoculated with a Barley stripe mosaic virus (BSMV)‐based silencing system and assayed by qRT‐PCR. (F, G) RNA expression of pathogenesis‐related genes TaPR2 (F) and TaPR10 (G) analysed by qRT‐PCR in BSMV:00‐, BSMV:TaBON1‐ and BSMV:TaBON3‐inoculated plants at 28 °C. Expression was normalized by the expression level in the control (BSMV:00). TaActin was used as the internal control. Similar results were seen in all three biological replicates, and one biological replicate with three technical repeats is shown. Asterisks indicate statistically significant differences in comparison with the control at P ≤ 0.01 (Student's t‐test).