Figure 5.

Proline iminopeptidase (PIP) is a new type III effector. (A) The calmodulin‐dependent adenylate cyclase (Cya) activity was determined by testing cyclic adenosine monophosphate (cAMP) concentration after 10 h of infiltration of bacterial strains into Arabidopsis plant leaves. Values were normalized by the protein contents in each sample. ΔhrcV is a type III secretion‐deficient mutant. The data were repeated at least three times. (B) The expression assay of Cya‐tagged fusion protein in bacterial cells and culture supernatants through western blotting with anti‐Cya mouse monoclonal antibody. ND, not detected.