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. 2017 May 19;19(4):801–811. doi: 10.1111/mpp.12560

Figure 4.

Figure 4

Induction of the defence response by XacPNP, and AtPNP‐A expression pattern, on Pseudomonas syringae pv. tomato (Pst) infection. (a) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) of Arabidopsis thaliana genes related to the defence response (see text for gene definitions). RNA was extracted from leaves infiltrated with 5 µm XacPNP at 6 h post‐inoculation (hpi). Bars indicate the expression levels of the indicated gene relative to the control treatment. As control, 5 µm 6 × His‐Trx was used (His, histidine; Trx, thioredoxin). (b) Enhancement of the defence response mediated by XacPNP. Quantification of Pst growth at 0, 3 and 6 days post‐inoculation (dpi) in leaves pre‐infiltrated with 5 µm XacPNP and 6 × His‐Trx as control. CFU, colony‐forming unit. (c) qRT‐PCR of AtPNP‐A and PR1 in A. thaliana leaves infected with Pst at 0, 2 and 4 dpi. Values are the means of three biological replicates with three technical replicates each. Error bars indicate standard deviations. The data were analysed by analysis of variance (ANOVA) (P < 0.05).