Table 2.
Presence of restriction modification and clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas) systems in Erwinia amylovora strains
| Strain ID | Phylogenetic group* | CRISRP group† | Presence of restriction modification systems‡ | Presence of cas1–cas5 | Number of CRISPR1 repeats§ | Number of CRISPR2 repeats§ |
|---|---|---|---|---|---|---|
| La637 | Epidemic | I | Yes | Yes | 38 | 23 |
| La635 | Epidemic | I | Yes | Yes | 28 | 23 |
| La636 | Epidemic | I | Yes | Yes | 36 | 23 |
| Ea110 | Epidemic | I | Yes | Yes | 37 | 23 |
| MASHBO | Epidemic | I | Yes | Yes | 37 | 26 |
| NHSB01‐1 | Epidemic | I | Yes | Yes | 28 | 26 |
| Ea266 | Epidemic | I | Yes | Yes | 10 | 22 |
| UT5P4 | Epidemic | II | Yes | Yes | 36 | 35 |
| ATCC49946 | Epidemic | I | Yes | Yes | 28 | 32 |
| 01SFR‐BO | Epidemic | I | Yes | Yes | 37 | 34 |
| CFBP1430 | Epidemic | I | Yes | Yes | 28 | 34 |
| ACW56400 | Epidemic | I | Yes | Yes | 37 | 34 |
| CFBP 1232 | Epidemic | I | Yes | Yes | 36 | 32 |
| CFBP2585 | Epidemic | I | Yes | Yes | 37 | 34 |
| Ea356 | Epidemic | I | Yes | Yes | 36 | 36 |
| WSDA87‐73 | Epidemic | I | Yes | Yes | 36 | 34 |
| VTBL01‐1 | Epidemic | I | Yes | Yes | 30 | 35 |
| VTDMSF02 | Eastern | ND | Yes | Yes | 81 | 55 |
| CTMF03‐1 | Eastern | ND | Yes | Yes | 84 | 55 |
| MAGFLF‐2 | Eastern | ND | Yes | Yes | 55 | 40 |
| CTST01‐1 | Eastern | ND | Yes | Yes | 82 | 55 |
| CTBT3‐1 | Eastern | ND | Yes | Yes | 94 | 25 |
| CTBT1‐1 | Eastern | ND | Yes | Yes | 94 | 25 |
| NHWL02‐2 | Eastern | ND | Yes | Yes | 90 | 25 |
| MANBO2‐1 | Eastern | ND | Yes | Yes | 91 | 29 |
| RISTBO01‐2 | Eastern | ND | Yes | Yes | 94 | 58 |
| OR6 | Western 1 | III | Yes | Yes | 97 | 49 |
| LA092 | Western 1 | III | Yes | Yes | 56 | 49 |
| OR1 | Western 1 | III | Yes | Yes | 98 | 49 |
| CA3R | Western 2 | ND | Yes | Yes | 23 | 49 |
*Epidemic, Widely Prevalent clade; Eastern, Eastern U.S. clade; Western 1, Western U.S. 1 clade; Western 2, Western U.S. 2 clade.
†The CRISPR groups were determined according to the groups named by Rezzonico et al. (2011). ND indicates that the strain was not included in the previous CRISPR study and the CRISPR pattern did not match any of the groups determined in that study.
‡Presence of 13 restriction modification system genes, EAM_0060, EAM_0061, EAM_0062, EAM_0280, EAM_0862, EAM_0863, EAM_0864, EAM_2079, EAM_2080, EAM_3033, EAM_3237, EAM_3405, EAM_3406, identified by the REBASE database (Roberts et al., 2015).
§Number of CRISPR repeats was calculated by CRT1.1 (Bland et al., 2007).