Skip to main content
. 2017 Nov 16;19(5):1196–1209. doi: 10.1111/mpp.12597

Figure 7.

Figure 7

AvrP123 shows transcriptional activation in yeast. (a) AvrP/AvrP123 and P/P2 interaction assays in yeast. Avr and R gene constructs were fused to either a GAL4 activation domain (AD) or a binding domain (BD) in vectors that enable yeast transformants to grow in minimal medium lacking tryptophan and leucine (–WL), respectively. As a result of the interaction between AD and BD fusion proteins, the HIS3 reporter gene is activated, enabling the yeast to grow in medium lacking histidine, tryptophan and leucine (–HWL). L6 and AvrL567 constructs were used as positive controls. (b) BD‐AvrP123 construct activates yeast growth in the absence of AD constructs in medium lacking histidine and tryptophan (–HW).