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. 2017 May 3;19(3):664–676. doi: 10.1111/mpp.12550

Figure 1.

Figure 1

The susceptibility of the A17 genotype to Aphanomyces euteiches is decreased on nitrate‐deficient (NØ) medium. (A) Susceptibility to A. euteiches as scored by the percentage of symptomatic tissues [stem necrosis at 21 days of culture and root browning at 10 days post‐inoculation (dpi), see Experimental Procedures]. (B) Quantification by enzyme‐linked immunosorbent assay (ELISA) of A. euteiches in root extracts. FW, fresh weight. (C) Relative expression of AeTUB using MtEF1 as reference gene. Plants were cultivated in vitro for 3 weeks on complete medium or NØ medium and inoculated with A. euteiches. For ELISA, the background signal from control non‐inoculated roots was subtracted from the signal detected in inoculated roots. Error bars indicate standard errors (n = 16–20 for symptoms; n = 4–6 biological replicates for ELISAs and AeTUB expression) and asterisks indicate significant differences (P < 0.05). Data from a representative experiment out of four independent experiments are shown.