Figure 7.

Root contents on complete medium (A) and nitrate‐deficient (NØ) medium (B) for the six specific amino acids whose accumulation is significantly induced by Aphanomyces euteiches in NØ‐IS genotypes on complete medium. Amino acid quantification was performed on four genotypes (F83005.5, SA022322, A17 and DZA045‐6). Plants were cultivated in vitro for 10 days on complete medium or NØ medium and inoculated (+) or not (–) with A. euteiches. Amino acids were measured on three biological replicates. The percentage of the average level of each amino acid is displayed for the four selected genotypes in each condition. For each amino acid, the average levels were determined as the mean levels of the amino acid in all conditions, and were equal to 7.5 µmol/g dry weight for glutamine (Gln), 1.6 µmol/g dry weight for arginine (Arg) and lysine (Lys), 0.8 µmol/g dry weight for tyrosine (Tyr), 0.3 µmol/g dry weight for methionine (Met) and 1.3 µmol/g dry weight for tryptophan (Trp). The colour scale limits were 30% (blue) and 230% (yellow) of the root amino acid average content. Absolute values for amino acid contents are displayed in Table S2 (see Supporting Information). NØ‐IS, genotype displaying NØ‐induced susceptibility; NØ‐IR, genotype displaying NØ‐induced resistance.