Plant elicitor peptide (Pep) seed treatments limit nematode reproduction on soybean. (A) Reproduction by the root‐knot nematode (Meloidogyne incognita) was compared by measuring the number of egg masses per gram of dry root mass at 7 weeks after inoculation (n = 10 for H2O, n = 11 for GmPep1, n = 11 for GmPep2, n = 6 for GmPep3). (B) Reproduction of the soybean cyst nematode (Heterodera glycines) was also compared on plants that had received seed treatments with GmPep1, GmPep2 or GmPep3 (1 µm) or water by counting the number of cysts per plant at 2 months after inoculation (n = 15 for all treatments). For both nematode species, there were significant differences in reproduction amongst the treatment groups [one‐way analyses of variance (ANOVAs), P < 0.05]. Treatments labelled with asterisks are significantly different from the water‐treated control group according to Student's t‐tests (α = 0.05). Error bars represent the standard deviations (SDs).