Figure 2.

Relative expression of selected type III effector (T3E) genes in Ralstonia solanacearum. Total RNA was isolated from GF001 and prhO mutant (GF0094), and mRNA levels of representative T3E genes in hrp‐inducing medium were determined by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). serC was selected as reference for normalization. Normalized values of GF0094 were divided by those of GF001 and relative values (relative expression) are presented. Mean values of at least three independent trials were averaged and are presented with standard deviation (SD) (error bars). Statistical significance was assessed between normalized values of GF001 and GF0094. Significance level: *P < 0.05; **P < 0.01 (t‐test).