Figure 7.

Effect of PrhO on biofilm formation and swimming motility. (a) Biofilm formation. Left, OE1‐1 derivatives; right, GMI1000 derivatives. Twenty microlitres of bacterial suspension at an optical density at 600 nm (OD₆₀₀) of 0.1 were inoculated into 180 μL of fresh rich medium and kept at 28 °C for 24 h without shaking. After staining with 0.1% crystal violet, biofilm formation was quantified with the absorbance at 530 nm (A ₅₃₀) and normalized with OD₆₀₀. (b) Swimming motility. Three microlitres of bacterial suspension at OD₆₀₀ = 1.0 were dropped onto 0.3% agar plates and kept at 28 °C for 48 h. Swimming motility was quantified as the halo diameter in millimetres. hpi, hours post‐inoculation. Mean values of all results were averaged and are presented with standard deviation (SD) (error bars).