Expression of ICS and PAL genes in barley. The mRNA expression of HvICS (a), HvPAL1 (b), HvPAL3 (c), HvPAL6‐1 (d), HvPAL6‐4 (e) and HvPAL6‐5 (f). The expression of these genes was analysed in the wild‐type ‘Golden Promise’ at the heading stage. The tested organs were the flag leaf, penultimate leaf (leaf), peduncle (stem), sheath of the flag leaf (sheath) and inflorescence (spike). All measurements were normalized to the expression of Actin. Using SAS version 9.0 (SAS Institute Inc., Cary, NC, USA), comparisons were made using Duncan multiple range tests at a significance level of 0.05. Error bars represent the standard error of the mean (SEM).