Table 1.
Distribution of ripAX2 within the Ralstonia solanacearum species complex (RSSC) world collection as estimated by polymerase chain reaction (PCR) sequencing (A), and within the RalstoT3E database of genomic sequences as estimated by automatic annotation (B). The gene was considered to be present if annotated as functional (PROT), frameshifted (FS) or pseudogene (PS). Results are expressed in strain numbers (percentage). Sample distributions per state (presence/absence) and phylotype were compared using a χ 2 test.
| Phylotype | Absence | Presence | Total (% of the collection) | P value (χ 2 test)† |
|---|---|---|---|---|
| ||||
| I | 12 (18.2) | 54 (81.8) | 66 (72.5) | 0.017* |
| IIA | 5 (55.6) | 4 (44.4) | 9 (9.9) | 0.127NS |
| IIB | 10 (100.0) | 0 (0) | 10 (11.0) | <0.0001*** |
| III | 2 (33.3) | 4 (66.7) | 6 (6.6) | 0.938NS |
| Total | 29 (31.9) | 62 (68.1) | 91 | |
| P value (χ 2 test)‡ | 0.0002*** | 0.025* | ||
| ||||
| I | 13 (48.1) | 14 (51.9) | 27 (36.5) | 0.019* |
| IIA | 5 (83.3) | 1 (16.7) | 6 (8.1) | 0.445NS |
| IIB | 16 (66.7) | 8 (33.3) | 24 (32.4) | 0.811NS |
| III | 2 (100) | 0 (0) | 2 (2.7) | 0.342NS |
| IV | 15 (100) | 0 (0) | 15 (20.3) | 0.009** |
| Total | 51 (68.9) | 23 (31.1) | 74 | |
| P value (χ 2 test)‡ | 0.37NS | 0.05NS | ||
†The H0 tested is: ‘Respective frequencies of ripAX2 presence and absence within each phylotype are equal to their expected values based on the total frequencies’.
‡The H0 tested is: ‘Respective distributions of phylotypes within each ripAX2 state (presence, absence) are equal to their expected values based on the total frequencies’.
NS, not significant; *significant (0.05 > P ≥ 0.01); **highly significant (0.01 > P ≥ 0.001); ***very highly significant (P < 0.001).