Figure 3.

Cell aggregation results for Ralstonia solanacearum OE1‐1, the ralfuranone‐deficient mutant (ΔralA) and the complemented ΔralA mutant (ralA‐comp) strains. OE1‐1, ΔralA and ralA‐comp cells were incubated in one‐quarter‐strength M63 medium in wells of polyvinylchloride microtitre plates. ΔralA cells were also incubated in one‐quarter‐strength M63 medium supplemented with ralfuranones A, B, J, K or L at concentrations of 0.2–20 μm. The wells were stained with crystal violet. Asterisks indicate values that are significantly different from those of the ΔralA strain in apoplast fluid (P < 0.05, t‐test). OD, optical density.