Figure 7.

Expression of rpoN1, rpoN2 and rpoS in Ralstonia solanacearum strain OE1‐1 and the ralfuranone‐deficient mutant (ΔralA). Total RNA was extracted from cells grown in one‐quarter‐strength M63 medium [optical density at 600 nm (OD₆₀₀) = 0.3] (a) or in one‐quarter‐strength M63 medium containing 20 μm ralfuranones A, B, J, K or L (b). The rpoD gene was used as an internal control for quantitative real‐time polymerase chain reaction. The gene expression levels are presented relative to the rpoD expression level. The experiment was conducted at least twice using independent samples, with similar results. Results for a single representative sample are provided. Values are presented as the mean ± standard deviation of three replicates. Asterisks indicate values that are significantly different from those of OE1‐1 (a) and ΔralA (b) (P < 0.05, t‐test).