Figure 7.

Accumulation of 3 × flag‐tagged p24 and p24 mutant derivatives in infiltrated tissues at 3 days post‐infiltration (dpi) in the presence or absence of p19 protein. (A) Tests of RNA silencing suppression for flag‐tagged p24 and p24 mutant derivatives. Nicotiana benthamiana leaves were co‐infiltrated with pGD–GFP and flag‐tagged wild‐type (wt) p24 or its dysfunctional mutants 2A, 54A, 86A, 149A, 162/169/170H and 35/38/85/89H. Photographs were taken as indicated in Fig. 1A. (B) Assessment of the accumulation of flag‐tagged p24 and p24 mutant derivatives by western blot analysis using a flag antibody. Plus p19, in the presence of Tomato bushy stunt virus (TBSV) p19.