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. 2017 Feb 28;19(2):355–368. doi: 10.1111/mpp.12525

Figure 8.

Figure 8

Effects of p24 on protein and transcript levels of AGO1. (A) p24 does not promote argonaute 1 (AGO1) of Arabidopsis thaliana (AtAGO1). Nicotiana benthamiana leaves were co‐infiltrated with 6 × myc‐tagged AtAGO1 and pGD or pGD–p24. The accumulation of AtAGO1 was evaluated by western blotting in agroinfiltrated regions harvested at 2, 4, 5 and 6 days post‐infiltration (dpi) using anti‐myc antibody. (B) Effects of p24 and its dysfunctional mutant on the transcript level of N. benthamiana AGO1 (NbAGO1). Nicotiana benthamiana leaves were agroinfiltrated with pGD–p24–flag, pGD–2A–flag or pGD–GFP. Total RNA and protein were extracted from agroinfiltrated regions harvested at 3 and 5 dpi for the detection of the mRNA expression level of NbAGO1 by quantitative real‐time reverse transcription‐polymerase chain reaction, testing the accumulation of p24 and 2A by western blotting. Bars represent grand means ± standard deviation (SD).