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. 2018 Nov 2;19(12):2603–2622. doi: 10.1111/mpp.12732

Figure 6.

Figure 6

In vivo interaction of Ustilago hordei effectors with Hordeum vulgare target proteins via a yeast two‐hybrid (Y2H) system. Interaction of pGBKT7‐UHOR_02413 and pGADT7‐HvHMA, pGBKT7‐UHOR_02413 and pGADT7‐HvDUF866, and pGBKT7‐UHOR_02700 and pGADT7‐HvTLP4 was confirmed via Y2H assay. The empty vectors were used as negative controls for the Saccharomyces cerevisiae AH109 strain. Different dilutions (100–10–3) of each sample were dropped on low‐ [–leucine (‐Leu) and –tryptophan (‐Trp)] and high‐ [‐Leu, ‐Trp, –adenine (‐Ade) and –histidine (‐His)] stringency plates. The photographs were taken after 3–4 days of incubation at 28 ºC. Growth on high‐stringency plates indicates interaction.