Figure 4.
T&HS provokes dynamic changes in lymphocyte subsets which are cell specific, compartment specific and time dependent. Flow cytometry was used to examine the lymphocyte subset populations at 6 and 24 h following injury. Blood, spleen and bone marrow were the compartments selected for examination. Changes in peripheral blood populations of NK 1.1+ CD3– cells were observed at 6 h, while NK1.1+ CD3+, γδTCR+ CD3+ cells, CD3+ CD4+, and CD3+ CD8+ cell populations remained unchanged. In spleen, a decrease in the population of NK 1.1+ CD3–, NK1.1+ CD3+, CD3+ CD4+, and CD3+ CD8+ was observed by 6 h, while γδTCR+ CD3+ cells remained broadly unchanged. In bone marrow, the NK 1.1+ CD3– cell population fell by 6 h while the other cell populations were seen to increase. Examination of lymphocyte sub-set populations revealed dynamic shifts within the immune compartments, principally within the first 6 h. Control (C) denotes a naïve subject of the same strain and age. Data are presented as mean (SEM) absolute cell counts × 106 (per ml of blood, per spleen or per both hind limbs) and tested using Kruskal Wallis with Dunn's multiple comparison of columns, *denotes p < 0.05.