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. 2019 Jul 11;10:1501. doi: 10.3389/fimmu.2019.01501

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Copyright © 2019 Manson, Hoffman, Chen, Ramadan and Billiar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Innate-like cells in blood change their phenotype and function during the first 6 h following T&HS. To determine whether the innate-like cells in this study changed their phenotype after T&HS, flow cytometry was conducted on blood and spleen taken at several sample points during the protocol. This revealed dynamic changes in all three cells, within the first 6 h of injury. Percentage blood phenotype: percentage of CXCR3, MHC II, and NKG2D positive cells. Data are presented as median (min-max) and tested with Kruskal Wallis and Dunns multiple comparison test, *denotes p < 0.05.