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In vitro assays reveal a loss of flagella‐mediated swimming motility in the mutant compared with the wild‐type strain. Pcb1692 cells cultured overnight in Luria–Bertani (LB) broth and cells harvested from Pcb1692‐inoculated stems at 6, 24 and 72 h post‐inoculation (hpi) were obtained using a sterile pipette and stabbed in the centre of 0.4% polygalacturonic acid (PGA) plates. Photographs were taken after 48 h.
