Figure 1.

Cysteine‐rich effector protein production in Escherichia coli. (a) Coomassie brilliant blue (CBB)‐stained sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analysis of total protein from E. coli expression experiments. S and B denote SHuffle and BL21 strains, respectively. Arrows indicate the expected migration of the overexpressed proteins. (b) Analysis of histidine‐tagged protein derived from crude cell lysates using BLItz (FortéBio). The binding rate of the histidine‐tagged protein to the sensor, which is directly proportional to the protein concentration in the sample, was measured. (c) CBB‐stained SDS‐PAGE analysis of soluble protein purified in small‐scale nickel affinity purification experiments, labelled as in (b). *Samples analysed by tryptic digest mass spectrometry.