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. 2016 Apr 12;18(1):141–151. doi: 10.1111/mpp.12385

Figure 2.

Figure 2

Small cysteine‐rich (SCR) effector purification and secondary structure analysis. (a) Circular dichroism (CD) analysis of the purified AvrP. (b) Size exclusion chromatography (SEC) profile of AvrP on Hiload 26/600 Superdex 75 PG. Coomassie brilliant blue (CBB)‐stained sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of protein from the identified peak (inset). (c) Predicted secondary structure analysis output from PSPIRED (http://bioinf.cs.ucl.ac.uk/psipred/) (Buchan et al., 2013; Jones, 1999). (d–f) As for (b, a, c), but performed with SnTox1. (g) SEC profile of SnTox3 on 10/300 Superdex 75HR. CBB‐stained SDS‐PAGE of protein from the identified peak (inset). (h, i) As for (a, c), but performed with SnTox3. (j) The percentage of secondary structure elements estimated using CD analysis programs CDSSTR, CONTINLL and SELCON3. CDSSTR failed to estimate the secondary structure elements of AvrP (N/A).