Table 1.

Small cysteine‐rich (SCR) effectors studied.

Protein	Organism	Molecular weight (kDa)	Residues	Cysteines	Predicted localization	ScanProsite*	Phyre2†
AvrP	Melampsora lini	9.5	88	10	Intracellular	Kazal serine protease inhibitor family signature	PHD domain (zinc binding)
AvrP123	Melampsora lini	10.3	94	10	Intracellular	Kazal serine protease inhibitor family signature	100.0
AvrP4	Melampsora lini	7.3	67	6	Intracellular	No hit	–
SnTox1	Parastagonospora nodorum	10.3	100	16	Unknown	No hit	100.0
SnTox3	Parastagonospora nodorum	23.6	209	6	Unknown	No hit	100.0
avrM‡	Melampsora lini	27.1	235	0	Intracellular	Not applicable	Not applicable

*ScanProsite (http://prosite.expasy.org/scanprosite/) hits matched by miniprofile (Hulo et al., 2008).

†Phyre2 (http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id = index) hits displayed with confidence levels >80%.

‡Positive control for protein expression. The sequences of the constructs used are detailed in Table S2 (see Supporting Information).