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Secretion of the Ss‐Rhs1 (Sclerotinia sclerotiorum Rearrangement hotspot repeat 1) protein. The Ss‐Rhs1‐Flag‐engineered strain was cultured with shaking for 4 days and the proteins in the hyphae and culture filtrate were extracted. The proteins were then subjected to Western analysis using an anti‐Flag (top) or anti‐Actin (bottom) antibody.
