Figure 4.

Real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) analysis of the Ss‐Rhs1 (Sclerotinia sclerotiorum Rearrangement hotspot repeat 1) gene transcript during different sclerotial development stages (A) and after contact with Arabidopsis thaliana (dark columns) and growing on potato dextrose agar (PDA) plates (grey columns) (B). The quantity of Ss‐Rhs1 cDNA in each sample was normalized to that of tub1 cDNA. The relative abundance of Ss‐Rhs1 cDNA in the stage of hyphal growth or in mycelium inoculated on PDA or plants at 0 h was set as unity. Bars indicate the standard error. The analyses were repeated three times. Gene expression levels in different replicates showed similar trends. One replicate is shown.