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. 2016 Sep 8;18(8):1052–1061. doi: 10.1111/mpp.12459

Figure 5.

Figure 5

Construction of the Ss‐Rhs1 (Sclerotinia sclerotiorum Rearrangement hotspot repeat 1) gene RNA interference (RNAi) vector and phenotype of Ss‐Rhs1‐silenced strains. (A) Construction of the Ss‐Rhs1 RNAi vector pSIRHS1. (B) Relative expression level of Ss‐Rhs1 in different isolates containing pSIRHS1, as well as in the wild‐type strain, as determined by real‐time reverse transcription‐polymerase chain reaction (RT‐PCR). The quantity of S. sclerotiorum tub1 cDNA normalized different samples. The relative expression level of Ss‐Rhs1 in the wild‐type strain was set to unity. Bars indicate the standard error. (C) Phenotypes of the wild‐type strain, Sirhs‐66, Sirhs‐68 and Sirhs‐93. The strains were grown on PDA medium for 15 days.