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. 2016 Jun 10;18(3):347–362. doi: 10.1111/mpp.12401

Figure 8.

Figure 8

VdNRS/ER (NRS/ER, nucleotide‐rhamnose synthase/epimerase‐reductase) deletion strains are depleted in uridine diphosphate (UDP)‐rhamnose. Gas chromatography‐mass spectrometry (GC‐MS) analysis of alditol acetate derivatives of the glycosyl residues from conidiospore polysaccharides. (A) Total ion count chromatogram of alditol acetate‐derived standard rhamnose (Rha) and mass spectrum (inset) showing fragmentation pattern fingerprints with m/z 129, 171, 231, 303. (B) Polysaccharides from wild‐type Verticillium dahliae (WT) conidiospores display a peak eluting at 16.10 min that has the same retention time as rhamnose standard. (C) VdNRS/ER deletion strain (Δ6010‐1) lacks a peak eluting at 16.10 min. (D) The VdNRS/ER deletion strain complemented with the native VdNRS/ER gene (Comp‐2) displays a peak eluting at 16.10 min that has the same retention time as the rhamnose standard.